Saturday, October 27, 2007

Microarray in general

### cDNA microarray - 2 color

Cy5: Red (experimental, mutant)
Cy3: Green (reference, wild-type)

M = log2(R/G)
A = {log2(R) + log2(G)}/2

### "Long" oligo arrays
- two color and double-stranded
- 60-80 bp long

### Affymetrix arrays
- one color array and single stranded
- 25 bp

# probe: material that is purposedfully places on the array before the experiment
# target: the material that is gathered from a sample
# hybridization: target material is put on array, then targets stick to complementary probes

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