### cDNA microarray - 2 color
Cy5: Red (experimental, mutant)
Cy3: Green (reference, wild-type)
M = log2(R/G)
A = {log2(R) + log2(G)}/2
### "Long" oligo arrays
- two color and double-stranded
- 60-80 bp long
### Affymetrix arrays
- one color array and single stranded
- 25 bp
# probe: material that is purposedfully places on the array before the experiment
# target: the material that is gathered from a sample
# hybridization: target material is put on array, then targets stick to complementary probes
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment